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With KeyMacro, you can change your browser's proxy server settings directly from the system tray. All you need to do is select your desired proxy server, click the 'OK' button and the changes will be applied automatically!Investigations of the mechanism of nitric oxide production in reperfusion injury in rat hearts.
The aim of this study was to test the hypothesis that oxygen free radicals, in addition to xanthine oxidase, are involved in nitric oxide (NO) production in reperfusion injury of rat hearts. We used two isoforms of xanthine oxidase (XO), the inducible (iXO) and constitutive (cXO), as well as the nitric oxide synthase inhibitor, L-N(6)-(1-iminoethyl)lysine, and H2O2 scavenger, catalase. Rats received either iXO, cXO, or L-N(6)-(1-iminoethyl)lysine, and hearts were subjected to 25 min of global ischemia and 30 min of reperfusion. In addition, the hearts were treated with catalase 1 min before the start of the ischemia. Cardiac NO production was determined from the heart-to-blood ratio of total cGMP using radioimmunoassay. NO levels were significantly higher in the iXO and L-N(6)-(1-iminoethyl)lysine groups than in the sham group. NO production was inhibited by the iXO and L-N(6)-(1-iminoethyl)lysine treatments, and increased in the catalase group. The cXO group showed a higher NO production than the sham and iXO groups, which was inhibited by L-N(6)-(1-iminoethyl)lysine. The cardiac NO production in the sham group was decreased by L-N(6)-(1-iminoethyl)lysine. H2O2 levels were higher in the cXO group than in the sham and iXO groups, which were increased by L-N(6)-(1-iminoethyl)lysine. These results suggest that oxygen free radicals such as hydroxyl and superoxide anions are involved in NO production in reperfusion injury.MacDonald House 70238732e0 betosva
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[caption id="attachment_1705" align="aligncenter" width="4533"] ColorGrabber 9.1.[/caption]
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